Elisa Assay

When a positive + result occurs ELISA, then one could conclude that...

• a + result always means illness

• it is a false + and no illness

• the antibody is present

What is a primary antibody?

• the second antibody used to detect foreign particle

• particle produced by the antigen

• the first antibody used to detect foreign particle

• second cell produced by the macrophage

Why is a positive and negative control necessary in the setup of ELISA?

• positive controls are equal to negative controls

• ELISA is subject to errors; if controls fail the results are untrustworthy

• ELISA is a well run test that typically does not need controls

• negative and positive controls are needed to exclude all results

Why is it necessary to wash the samples repeatedly?

• it is important to keep the lab wells clean

• it is important to wash away all bound and unbound antibodies from the wells

• it is important to wash away the unbound antibodies

• washing the wells is just solid lab practice

A serial dilution is

• the step wise saturation of a substance in solution

• the step wise dilution of a substance in solution

• a series of sequential dilutions used to increase a dense culture of cells to a more usable concentration.

• a dilution that increase by a factor of 1/10 every step

What does the symbol μL stand for?

• nanoliters

• microliters

• muliters

• milliters

From what cells do antibodies originate?

• b cells

• prison cells

• t cells

• red blood cells

The basic 4 steps of the ELISA are:

bind sample to well

add primary Ab and wash

add secondary Ab/enzyme and wash

add substrate

• true

• false

what does ELISA stand for

• enzyme linked immune sorbent assay

• enzyme linked independent sorbent assay

• enzyme linked immune system assay

• enzyme linked immune stain association

Why do you assay your samples in duplicates?

• To act as a control of the assay

• To test for pipetting errors

• Reproducibility of results

• all of the above