Molecular Sieve Chromatography

In size exclusion chromatography, separation is based on ...

• molecular shape

• molecular size

• specific reactions

• molecular charge

In Size exclusion chromatography,...

• larger molecules move more slowly through the column

• smaller molecules move more quickly through the column

• larger molecules move more quickly through the column

• Large and small molecules move at the same rate through the column

A fraction is..

• the liquid added to the top of the column

• all the liquid leaving the bottom of the column

• a timed sample of the liquid leaving the bottom of the column

• part of the gel in the column

The eluant is

• the fluid used to move the sample through the column

• the gel in the column

• the molecules of interest

• the stationary phase

The hydrodynamic volume represents ...

• the size of the molecule of interest

• the size of the molecule of interest and bound water

• the size of the gel beads

• the size of the pores in the gel beads

Which of the following can be used for size exclusion chromatography

• agarose

• dextran

• polyacrylamide

• all of the above

In size exclusion chromatography, a wide separation between components as they exit the column represents

• poor resolution

• good resolution

• unresolved components

• none of the above

In size exclusion chromatography, the size exclusion limit provides information on

• the bead's size

• the pore size

• the length of the column

• the volume of eluant.