Liquid/Gas Chromatography

Chromatography is used to separate the components of a mixture and can be carried out in a range of different ways.

a) A suitable example of a ‘carrier gas’ in gas chromatography is

• Chlorine

• Nitrogen

• Steam

• Not possible to state, as there should be a vacuum

(b) Separation is achieved in gas chromatography due to the components in the mixture having different

• Interactions with the stationary phase.

• Interactions with the mobile phase.

• Colours

• Solubility in the moving solvent.

Which gas is the least suitable as a carrier gas in Gas-Liquid Chromatography?

• Argon

• Carbon Dioxide

• Oxygen

• Nitrogen

Chromatography may be used to separate mixtures of amino acids. This is best explained by the fact that different amino acids have different interactions with

• the mobile phase only

• the stationary phase only

• the mobile phase and the stationary phase

• ninhydrin

In one type of HPLC, the stationary phase is non-polar and a polar solvent is used as the eluent. Which of the following would travel through the chromatography column most quickly?

• Tetrachloromethane

• Chloromethane

• Iodomethane

• Hexane

In HPLC (High Performance Liquid Chromatography) which of these factors does not affect the time taken for a component to pass through the column?

• Type of detector

• Material of stationary phase

• Particle size of stationary phase

• Temperature of column

In gas chromatography, the time taken for a substance to travel through the column is called

• Reference time

• Retention time

• Separation time

• Travelling time

Why is GC-MS an important analytical technique in science?

• Allows unknown substances to be identified

• Quantifies the amount of known chemicals

• Characterizes the boiling point of a compound

• Determines the volatility of a compound

Fill in the blank : The more soluble the colour in water __________ the colour is carried

• The further up the paper

• the more water will soak up the paper

• more spots of mixture are placed on pencil line

• The lower down the paper

Which of the following columns are not used in HPLC

• Analytical column

• Separation column

• Guard column

• Capillary column