Agarose Gel Electrophoresis

DNA fingerprinting works because

• Most genes are dominant
• All organisms contain RNA
• The most important genes are different among most people
• No two people, except identical twins, have exactly the same DNA 

Tags

NGSS.HS-LS3-2

What is the purpose of gel electrophoresis?

• helps cut DNA
• count the genes in DNA
• separates DNA based on size
• allows for an exact replicated organism

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing
• It restricts the number of base pairs that can be tested in a sample
• It makes the testing simpler by moving the strands into the gel faster
• It charges the DNA strands

A student performed a gel electrophoresis experiment. The results are represented in the diagram

below. Compared to the fragments at the top of the gel, the fragments at the lower end are

• larger, and move slower

• larger, and move faster

• smaller, and move faster

• smaller, and move slower

Once DNA has been extracted from the nucleus of a cell, it is exposed to ______ ______ that act like biochemical scissors to cut the DNA.

• restriction enzymes

• gel agarose

• clotting factors

• electric shock

Gel electrophoresis enables scientists to

• separate DNA fragments.
• combine DNA fragments.
• count the genes in DNA.
• insert DNA in cells.

During gel electrophoresis, DNA moves from the ___ end to the ___ end.

• positive, negative
• negative, positive

How are buffers used in Electrophoresis?

• Conduct electricity

• Remove salts

• Add extension energy

• Dissolve excess DNA

What type of bonds hold the two strands of DNA together?

• Hydrogen bonds
• Covalent bonds
• Dipole-Dipole bonds
• Ionic bonds