IB Biology Topic 3.5

A special protein that targets a specific base sequence and cuts DNA into smaller fragments. (a)  

• Restriction Endonuclease (Enzyme)

• PCR

• DNA Polymerase

• Helicase

What is the term given to asymmetrical ends of DNA after being cut by a restriction enzyme?

• Blunt Ends

• Sticky Ends

• Ligated Ends

• Restrictive Ends

This biomolecule originates from bacterial DNA and has the ability to cut DNA....

• Helicase

• Ligase

• Restriction Endonuclease (Enzyme)

• DNA Polymerase

The unpaired nucleotides produced by the action of restriction enzymes are referred to as.....

• sticky end.

• base sequence.

• single strands.

• restriction fragments.

The recognition sites for restriction endonucleases (enzymes) are usually...

• alliterations

• pallindromes

• acronyms

• analogies

Restriction Endonuclease (Enzyme) are isolated from....

• proteins

• bacteria

• viruses

• bacteriophages

Restriction Endonucleases (enzymes) were primarily used as....

• defense by protozoa against antibiotics

• tools by viruses to cleave bacterial genome

• defense by bacteria against bacteriophages viruses

• tools to cleave DNA during replication

Which restriction endonucleases (enzymes) from above make blunt cuts? (2)

• Alul

• HaeIII

• BamHI

• HindIII

• EcoRI

How many fragments resulted from the cut above?

• 1

• 2

• 3

• 4

How many base pairs (bp's) make up each fragment?

• 11

• 14

• 7

• 22

• 28