BIOTECHNOLOGY PRINCIPLES AND PROCESSES

Who gave the term biotechnology?

• Karl Erkey

• Edward Jenner

• Robert Koch

• Louis pasture

Restriction endonucleases where isolated for the first time by

• Robert Brown

• Karl Erkey

• W. Arber

• F. Griffith

In EcoRI, what does 'co' stand for

• Genus

• Species

• Kingdom

• Phylum

What is the recognition sequence and site of cleavage for restriction enzyme Eco R1?

• 5'GGATCC3'

• 5'GAATTC3'

• 5'AAGCTT3'

• 5'CCCGGG3'

A sequence from where replication starts

• Selectable marker

• Bacteriophage

• Vector

• Origin of replication

Metals used for coating DNA fragment in wireless stick method are

• Gold and iron

• Gold and platinum

• Tungsten and magnesium

• Tungsten and gold

Enzyme used to dissolve the bacterial cell walls

• Cellulase

• Chitinase

• Protease

• Lysozyme

The first step in the process of recombinant DNA technology

• Cutting and ligation of DNA at specific locations

• Amplification of gene of interest using PCR

• Isolation of the genetic material or DNA

• Insertion of recombinant DNA into the host cell or organism

Full name of PCR

• Polymerase chain reaction

• Polymerase chemical restriction

• Polyethylene glycol

• Poli cystic recognition

Who invented PCR?

• Karl Erkey

• Robert Koch

• Kary Mulis

• Avery, McLeod, McCarty