Electrophoresis & DNA Profiling

What is the function of a primer?

• To identify the particular region of DNA to be copied by PCR.
• To copy DNA.
• To create DNA nucleotides.
• To maintain the temperature of the PCR reaction.

Why does PCR require a specific type of polymerase?

• Taq polymerase can withstand the range of temperatures in PCR without denaturing.
• Taq polymerase is the most inexpensive type of polymerase.
• Taq polymerase is the most common type of polymerase.
• A specific type of polymerase is unnecessary.

What is the purpose of gel electrophoresis?

• helps cut DNA
• count the genes in DNA
• separates DNA based on size
• allows for an exact replicated organism

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells
• farthest from the starting wells
• three quarters away from the starting wells
• it depends on how many fragments there are

DNA fingerprinting works because

• Most genes are dominant
• All organisms contain RNA
• The most important genes are different among most people
• No two people, except identical twins, have exactly the same DNA 

Which man is the father of this baby?

• Father 1
• Father 2
• Father 3
• None of them

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The parents of a new baby believe they brought the wrong child home from the hospital. Gel electrophoresis was performed using DNA samples from the parents and the child. A section of the gel

electrophoresis results is shown below. Which conclusion is valid based on the gel electrophoresis results?

• They have the correct child, because her genetic information is identical to that of the father.

  
  

• They have the wrong child, because her genetic information does not match that of either parent.

• They have the correct child, because her genetic information came from both parents.

  
  

• They have the wrong child, because her genetic information matches only that of the mother.

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Which is the likely 'sire' (dog's dad) of the given puppy?

• Sire A
• Sire B
• Sire C
• Sire D

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Based on these results whose blood was found in the blood stain at the crime scene?

• Bob
• Sue
• John
• Lisa

Which suspect is likely guilty?

• Suspect #1
• Suspect #2
• Suspect #3

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