High Performance Liquid Chromatography (HPLC) Quiz

What is the primary function of the pump in HPLC?

• To inject the sample

• To maintain stable flow of the mobile phase

• To detect the analytes

• To filter the mobile phase

Separation in liquid chromatography is based on different ___________ of the analytes.

What is the suitable sample types that can be analysed using gas chromatography?

• A mixture of thermally labile and volatile components.

• A mixture of thermally stable and volatile components.

• A mixture of thermally labile and non-volatile components.

• A mixture of thermally stable and volatile components.

Which type of chromatography is most widely used in liquid chromatography?

• Size Exclusion Chromatography

• Ion Exchange Chromatography

• Partition Chromatography

• Reversed Phase Chromatography

What is the purpose of a guard column in HPLC?

• To increase the pressure in the system

• To enhance the sensitivity of the detector

• To inject samples automatically

• To prevent clogging of the analytical column

Which of the following is NOT a type of detector used in HPLC?

• UV Absorbance

• Refractive Index

• Gas Chromatography

• Mass-Spectrometric

What is the main advantage of using HPLC?

• It requires large sample sizes

• It is only suitable for volatile compounds

• It provides greater sensitivity

• It is less expensive than other methods

What does the term 'isocratic elution' refer to?

• Using multiple columns in series

• Eluting with gas instead of liquid

• Using a constant solvent composition

• Changing the solvent composition continuously

Which component is responsible for removing dissolved gases from the mobile phase?

• Injector

• Pump

• Degasser

• Detector

What type of packing is used in size exclusion chromatography?

• Porous microparticles

• Alumina particles

• Non-porous glass beads

• Silica gel