Transcription and pre-mRNA processing (AP BIO)

Where does transcription take place in a eukaryote?

• nucleus

• endoplasmic reticulum

• cytosol

• ribosome

Where on the DNA does the enzyme responsible for transcription bind?

• promoter

• enhancer

• polyadenylation sequence

• start codon

Which enzyme is primarily responsible for the process of transcription?

• Topoisomerase

• Helicase

• DNA ligase

• RNA polymerase

What is the initial product of transcription in eukaryotic cells?

• mRNA

• tRNA

• pre-mRNA

• rRNA

Which of the following sequences signals the termination of transcription?

• enhancer sequence

• promoter sequence

• terminator sequence

• stop codon

Which molecules contains genetic information?

• DNA

• RNA

• mRNA

• protein

Tags

NGSS.MS-LS3-1

Which pre-mRNA processing step is important for initiating translation?

• Adding a poly-A tail

• RNA editing

• Splicing

• Adding the 7-methylguanosine cap

Contrast introns to exons:

Groups:

(a) Introns

,

(b) Exons

• found in mature mRNA

• play a crucial role in protein synthesis

• can regulate gene expression

• translated into proteins

• removed during RNA splicing

• can contain regulatory elements

• sequences that do not code for proteins

• found within DNA but not mRNA

• non-coding RNA that is removed

• coding sequences that are retained

• can be spliced together in different combinations

• may have roles in alternative splicing

Put the following steps of transcription in the correct order

• RNA polymerase pulls the double helix strands apart to reveal the nucleotide sequence of the template strand.

• mRNA leaves the nucleus through a nuclear pore.

• Transcription factors and RNA polymerase bind to the promoter region (TATA box).

• The termination sequence is transcribed and the RNA polymerase falls off.

• RNA nucleotides are added to the 3’ end of the growing strand of mRNA.