Biotechnology HW 9.1

What is the process called by which commercial volumes of a biotechnology product are produced, purified, and formulated?

• Biomanufacturing

• Genetic Engineering

• Chromatography

• Fermentation

Biomanufacturing starts with both cultures of transformed, transfected, or _______ cells.

• native

• engineered

• modified

• cloned

What is the process of extracting protein from a cell culture called?

• A) Harvest

• B) Recovery

• C) Purification

• D) Formulation

Fill in the blank: The method of harvesting depends on whether the protein is found within the cell (______) or outside of the cell (extracellular).

• A) Intracellular

• B) Extracellular

• C) Fermentation

• D) Formulation

What is the use of sonication in biotechnology?

• To separate proteins based on size

• To break open cells using high frequency sound waves

• To purify proteins using a column

• To adjust pH levels.

Column chromatography is a technique where a sample is passed through a column packed with a resin. This technique is used to separate molecules based on their ______.

• A) color

• B) size, shape, charge, or chemical nature

• C) temperature

• D) weight

What is the purpose of ultrafiltration in the pharmaceutical industry?

• To isolate a protein of interest

• To mix different proteins

• To degrade proteins

• To synthesize new proteins

In column chromatography, what accomplishes the separation of molecules in a mixture?

• The size of the molecules

• The charge of the molecules

• The affinity of the molecules

• The density of the molecules.

Which of the following is the first step in the biomanufacturing process?

• Purification

• Upstream Process

• Downstream Process

• Separation Technology