Biotechnology Fall Final

Front

What is recombinant DNA?

Back

DNA that results from the combination of DNA from two different species.

Front

What is the role of DNA ligase in gene cloning?

Back

It combines the isolated gene with plasmid DNA.

Front

Calculate the volume in mL of 50x TAE needed to make 2 L of 1x TAE electrophoresis buffer. Use C1V1=C2V2.

Back

40 mL.

Front

What is the purpose of the polymerase chain reaction (PCR)?

Back

To make many copies of a gene or other DNA segment.

Front

What role do restriction enzymes play in recombinant DNA technology?

Back

They cut DNA at specific sequences.

Front

Define biotechnology.

Back

The use of living systems and organisms to develop or make products.

Front

What is a plasmid?

Back

A small, circular piece of DNA that can replicate independently of chromosomal DNA.

Front

Explain the process of gene cloning.

Back

Gene cloning involves isolating a gene of interest and inserting it into a plasmid to produce multiple copies.

Front

What is the significance of the 1x TAE buffer in electrophoresis?

Back

It provides a conductive medium for the movement of DNA during electrophoresis.

Front

What is the function of a primer in PCR?

Back

Primers are short sequences of nucleotides that provide a starting point for DNA synthesis.