Sources and Tests of Pyrogens

What are pyrogens?

What are the main sources of pyrogens?

• virus

• bacteria

• fungi

• plant

What are the main pyrogen encountered in the pharmaceutical industry?

• Gram-negative bacteria  -> endotoxins

• Gram-positive bacteria

• virus

• fungi

What is the chemical structure of the endotoxins?

What tests are recognized by the USP?

• Pyogen Test conducted with rabbits

• BET – Bacterial Endotoxins Test

• LAL Test

• ATTEST

How the rabbit pyrogen test performed?

LAL = Bacterial Endotoxin Test also termed the…

How does the test work?