What is the primary purpose of DNA sequencing?

• To analyze RNA

• To clone genes

• To synthesize proteins

• To determine the precise sequence of nucleotides

Which method was developed by Frederick Sanger?

• Sanger Method

• Next Generation Sequencing

• Shotgun Sequencing

• Maxam-Gilbert Method

What does the Maxam-Gilbert method rely on?

• PCR amplification

• Enzymatic synthesis

• Chemical degradation

• Radioactive isotopes

Which chemical is used in the Maxam-Gilbert method for G nucleotides?

• Formic acid

• Piperidine

• Dimethyl sulphate

• Hydrazine

What is a disadvantage of the Sanger method?

• Cannot read >500bp

• Requires a large amount of DNA

• Is widely used

• Uses non-toxic chemicals

What is the main advantage of automated DNA sequencing?

• It is faster

• It requires less DNA

• It is less expensive

• It uses more toxic chemicals

What does NGS stand for?

• Next Generation Sequencing

• Nucleotide Gene Sequencing

• Nuclear Genome Sequencing

• Non-Genetic Sequencing

Which database is relevant to biotechnology and biomedicine?

• GenBank

• PubMed

• BLAST

• NCBI

What does BLAST help to identify?

• Cellular functions

• Gene families

• Protein structures

• Chemical compounds

What is a characteristic of the Sanger method?

• Uses toxic chemicals

• Cannot read more than 700 bases

• Requires large amounts of DNA

• Directly reads purified DNA