Jan-8-Discussion-1

Which of the following is a key step in RNA-Seq for analyzing transcriptomes?

• Direct sequencing of RNA without conversion to cDNA

• Extraction of total RNA followed by conversion to cDNA using reverse transcription

• Hybridization of cDNA to a microarray

• abeling cDNA fragments with fluorescent dyes before sequencing

Which of the following steps involves labeling synthesized cDNAs for visualization in DNA microarray analysis?

• Extracting mRNA from the target cell and generating cDNAs through reverse transcription

• Hybridizing labeled cDNAs to a DNA microarray to bind to complementary sequences

• Labeling synthesized cDNAs with fluorescent dyes to enable simultaneous analysis of control and test samples

• Using distinct fluorescent colors for labeling cDNAs from control and test samples before hybridization

What is the purpose of using a complementary probe using in situ hybridization?

• To break down the mRNA in the sample.

• To amplify the mRNA signal by repeated cycles.

• To bind specifically to the target mRNA, allowing its location to be visualized in the intact organism.

•  To replace the mRNA with a labeled DNA strand.

Why are DNA fragments attached to beads in next-generation sequencing (NGS)?

• To enable multiple DNA fragments to be sequenced on the same bead.

• To create a large number of identical copies of each DNA fragment through PCR.

• To enhance the fluorescence signal during sequencing.

• To allow selective washing of unincorporated nucleotides.

In a microarray experiment, why are cDNAs from control and test samples labeled with different fluorescent colors?

• To amplify the DNA signals for better detection.

• To differentiate between over- and under-expressed genes.

• To identify the exact sequence of each gene.

• To enable simultaneous comparison of gene expression between control and test samples on the same microarray.