Recombinant DNA Technology Quiz

What is the first step in recombinant DNA technology?

• Introduction of rDNA into host cells

• Isolation of the gene of interest

• Ligation of DNA fragments

• Transformation of the host cells

Which enzyme is primarily used to cut DNA at specific sites in recombinant DNA technology?

• DNA ligase

• DNA polymerase

• Restriction endonuclease

• RNA polymerase

What characteristic must a plasmid have to function as a cloning vector?

• It must replicate independently within the host

• It should not have a selectable marker

• It should be larger than 100 kilobases

• It must contain at least five different antibiotic resistance genes

In which year were the first recombinant DNA molecules generated?

• 1953

• 1973

• 1985

• 2001

What is the function of DNA ligase in recombinant DNA technology?

• To cut DNA at specific sequences

• To join DNA fragments together

• To separate DNA strands

• To amplify DNA

The process of introducing recombinant DNA into a host cell is known as:

• Transformation

• Cloning

• Transfection

• Electroporation

Which type of restriction enzyme is commonly used in recombinant DNA technology?

• Type I

• Type II

• Type III

• Type IV

What is a 'sticky end' in the context of recombinant DNA technology?

• A blunt end of a DNA fragment

• An overhanging single-stranded DNA region after cutting with certain enzymes

• A circular plasmid DNA without any cuts

• A double-stranded DNA region

The selectable marker in a plasmid vector is used to:

• Identify vectors that have not been taken up by host cells

• Cut the DNA at specific sites

• Allow plasmid replication in the host

• Identify cells that have successfully taken up the vector

Which method uses an electric field to introduce DNA into host cells?

• Microinjection

• Electroporation

• Transfection

• Transformation