Enzyme Kinetics Quiz

What does the Michaelis-Menten equation relate to?

• Enzyme concentration

• Initial reaction velocity and substrate concentration

• Inhibitor concentration

• Temperature and pH

What happens to the reaction rate as substrate concentration increases?

• It decreases linearly

• It remains constant

• It decreases exponentially

• It increases until enzymes are saturated

What is Km in the context of enzyme kinetics?

• The maximum velocity of the reaction

• The rate constant for product formation

• The substrate concentration at which the reaction rate is half of Vmax

• The total enzyme concentration

Which enzyme has a lower Km value?

• Both have the same Km

• Hexokinase

• Glucokinase

• Neither enzyme has a Km value

What is the effect of fasting on glycolysis?

• Increase in substrate concentration

• No effect

• Decrease in velocity

• Increase in velocity

What does a Lineweaver-Burke plot help determine?

• Reaction mechanism

• Enzyme concentration

• Vmax and Km

• Substrate specificity

What is the effect of enzyme concentration on reaction rate?

• It only affects Km

• It decreases the reaction rate

• It has no effect

• It increases the reaction rate for a fixed substrate concentration

What is the physiological role of hexokinase in RBCs?

• To store glycogen

• To convert glucose to urea

• To increase blood glucose levels

• To phosphorylate glucose at low blood glucose levels

What is the primary purpose of studying enzyme kinetics?

• To measure enzyme purity

• To determine the structure of enzymes

• To understand enzyme specificity and activity

• To analyze genetic variations

What happens to the reaction rate when all enzymes are saturated?

• It continues to increase

• It reaches a maximum

• It decreases

• It becomes linear