PCR fundamentals

PCR is an acronym for?

• Polymerase Chain Reaction

• Protein Complex Reaction

• Polymerase Chain Reading

In endpoint PCR when is the amplification of DNA analysed?

• Before the first cycle

• After the first cycle

• After the final cycle

What reporter dye is commonly used in real-time PCR to monitor amplification?

• Bromophenol Blue

• Crystal Violet

• SYBR Green

In gene expression analysis, what is the purpose of using a reference gene?

• To serve as internal controls

• To inhibit gene expression

• To amplify target genes

What is the function of a probe in a real-time PCR assay?

• To block amplification

• To bind to target sequences and generate fluorescence

• To degrade target DNA

Which type of PCR technique allows you to quantitate mRNA levels levels in a sample?

• End-point PCR

• Real-time PCR

• Reverse transcription PCR

In real-time PCR what does a low Cq value mean?

• High initial quantity of target

• Low initial quantity of target

• No DNA present

In real-time PCR what is the main purpose of the melt curve analysis?

• To determine the size of the PCR product

• To check for the specificity of amplification

• To increase the amplification rate

What is the role of reverse transcriptase in RT-qPCR?

• To amplify RNA

• To convert cDNA into RNA

• To convert RNA to cDNA

What is the main principle behind SYBR Green detection in real-time PCR?

• It binds to single-stranded DNA

• It inhibits DNA polymerase activity

• It fluoresces when bound to double-stranded DNA