CA_4 MmD

Which method is considered the 'gold standard' technique used by clinical laboratories to examine the molecular relatedness of bacterial isolates?

• AFLP

• MLST

• PFGE

• PCR fingerprinting

What is the purpose of Multi-locus variable number tandem repeat analysis (MLVA)?

• Characterize microbial communities

• Detect laboratory cross contamination

• Determine the number and length of variable number tandem repeats

• Identify single nucleotide polymorphisms

Which genotyping method uses the DNA sequence obtained from a highly variable single genetic locus to provide strain discrimination?

• SNPs typing

• MLST

• SLST

• T-RFLP

What is the main advantage of real-time broad-range PCR assays compared to conventional PCR?

• Ability to detect fungi

• Faster turnaround time

• Lower cost

• Higher analytical sensitivity

Which technology allows for simultaneous identification and characterization of a very large number of alleles of interest?

• T-RFLP

• Microarrays

• Deep Sequencing

• Broad-range PCR

What is the main advantage of barcoding pyrosequencing in high-throughput sequencing?

• Increased read length

• Simultaneous sequencing of multiple microbial communities

• Ability to generate thousands of sequence reads

• Reduced error rate

Which method is used to profile environmental communities and microbial communities associated with various body sites?

• Deep Sequencing

• MLST

• Microarrays

• T-RFLP

What is the main limitation of the T-RFLP technique?

• Amplification bias

• High cost

• Limited read length

• Low throughput

Which sequencing instrument is commonly used for community profiling due to the robustness of the chemistry?

• MLST

• Broad-range PCR

• Microarrays

• 454 FLX Titanium

What is the purpose of in silico prediction tools in microbial identification from T-RFLP data?

• To automate the T-RFLP technique

• To assist in microbial identification

• To identify operational taxonomic units

• To predict microbial communities