Chromatography Quiz

What is chromatography primarily used for?

• Separating molecules based on partitioning characteristics

• Quantifying the volume of a mixture

• Measuring the temperature of a solution

• Identifying components without a detector

Who first applied chromatography in 1903 for the separation of plant pigments?

• W. H. Freeman

• Daniel C. Harris

• M. S. Tswett

• Methane

What is the purpose of a detector in chromatography?

• To mix the mobile and stationary phases

• To adjust the flow rate of the mobile phase

• To identify chromatographically separated components

• To clean the column packing

What does the term 'eluent' refer to in chromatography?

• The stationary phase

• Fluid entering a column

• The detector response

• Fluid exiting a column

What does the Van Deemter Equation describe in chromatography?

• The color changes during elution

• The temperature range for optimal separation

• The chemical composition of the stationary phase

• Factors affecting the plate height and separation efficiency

Which gas is preferred over hydrogen in gas chromatography due to safety concerns?

• Helium

• Oxygen

• Nitrogen

• Carbon dioxide

What is the main advantage of using smaller particle size in liquid chromatography columns?

• Increased detector sensitivity

• Reduced column length

• Faster elution times

• Better separation efficiency

What does the number of theoretical plates indicate in chromatography?

• The temperature of the eluent

• Separation efficiency of the column

• The column's physical height

• The flow rate of the mobile phase

What is the purpose of the mobile phase in chromatography?

• Carrying the analyte through the column

• Staying in place inside the column

• Interacting with the stationary phase

• Detecting the separated components

How does the plate height affect the separation efficiency of a chromatographic column?

• Plate height affects the elution time only

• Plate height has no impact on separation

• Higher plate height leads to better separation

• Lower plate height leads to better separation