Cell culture techniques

1. Which of the following is used for maintaining a sterile environment during cell culture?

• Incubator

• Centrifuge

• Laminar flow cabinet

• Refrigerator

• Water bath

What is the primary purpose of using trypsin in cell culture?

• To feed the cells

• To stain the cells

• To detach cells from the culture dish

• To sterilize the culture medium

• To fix the cells

Which type of medium is most commonly used for mammalian cell culture?

• LB broth

• DMEM (Dulbecco's Modified Eagle Medium)

• Potato Dextrose Agar

• Nutrient Agar

• TSB (Tryptic Soy Broth)

What is the purpose of adding fetal bovine serum (FBS) to the culture medium?

• To inhibit cell growth

• To provide essential nutrients and growth factors

• To maintain pH balance

• To sterilize the medium

• To fix the cells

Which method is used to count the number of viable cells in a culture

• Western blotting

• Spectrophotometry

• Trypan blue exclusion assay

• Polymerase chain reaction

• Gel electrophoresis

Which of the following is a common method for cryopreserving animal cells?

• Using dry ice

• Freezing at -20°C

• Using liquid nitrogen

• Storing in a refrigerator

• Using a microwave

Which of the following is an indicator of contamination in cell cultures?

• Cell confluence

• pH of 7.4

• Cloudy medium and change in color

• High cell viability

• Stable cell growth

1. What is the role of antibiotics in cell culture?

• To provide growth factors

• To detach cells from the surface

• To prevent bacterial contamination

• To fix the cells

• To stain the cells

1. Which cell culture technique involves the growth of cells on a three-dimensional scaffold?

• 2D cell culture

• Organotypic culture

• Suspension culture

• 3D cell culture

• Monolayer culture

Which method is commonly used to measure cell proliferation?

• ELISA

• Flow cytometry

• MTT assay

• Northern blotting

• Southern blotting