Namita N Kashyap

Which type of molecules are separated by western blot based on size?

• Nucleotides

• Antibodies

• Proteins

• RNA

The stain which is used to visualize bands in a gel

• Ponceau

• TBST

• Coomassie Brilliant Blue

• Bromophenol blue

The blocking reagent used to visualize phospho-proteins

• Milk

• Non-fat milk

• Bovine serum albumin

• Water

Which was the primary antibody used for the purpose of this workshop?

• Beta-tubulin

• CD63

• Beta-HCG

• Beta-actin

Who coined the term "Western blot"?

• Neal Burnette

• Edwin Western

• George Stark

• George Laemmli

Which is the reducing agent used along with Laemmli buffer?

• SDS

• Beta-mercaptoethanol

• Bis-acrylamide

• TEMED

Purpose of glycerol in Laemmli buffer

• To give color to the sample

• To increase the density of the sample

• To give charge to the sample

• All of the above

pH of Tris base in resolving gel is

• 8.8

• 6.8

• 7.4

• 7.8

Percentage of acrylamide in stacking gel is

• 10%

• 5%

• 12%

• 0.5%

Identify the image

• Short plate

• Dummy plate

• Spacer plate

• Mirror