ELISA Background Notes Review

Secondary antibodies for ELISA are linked to ______________

• Substrates

• Enzymes

• HRP

• Catalyst

What is the name of the enzyme linked to the secondary antibody?

• Horseradish Peroxidase

• Primase

• Taq DNA Polymerase

• Ligase

Color that develops after the ___ minute incubation should not be considered in the assay results.

• 3

• 2

• 5

• 8

An agent that provokes an acquired immune response and is bound specifically by either antibodies or T cells.

• HRP

• TMB

• antibodies

• antigen

_________ are always run side by side with actual samples to make sure that the procedure is working correctly. They can resolve ambiguous results that occur due to human error or contaminated reagents. Must be included for ELISA to be valid.

• Control

• Constants

• Dependent Variable

• Independent Variable

Which of the following is a common blocking solution?

• 10X PBS

• Tween 20

• Fast Blast DNA Stain

• dH2O

In many diagnostic assays, results of the ELISA must be confirmed. Which test can confirm ELISA results?

• Southern Blot

• Western Blot

• Northern Blot

• Eastern Blot

To determine precise concentrations you will need to use __________ to get quantitative results.

• Vertical Gel Electrophoresis and Western Blot

• Gel Electrophoresis

• spectrophotometer/ microplate reader

• Autoclave.

When HRP oxidizes its substrate TMB, turning the assay solution ________.

• Green

• Blue

• Brown

• Purple

What field would use ELISA to detect viruses, mycotoxins in grains and corn, and track adulteration of non- GMO crops with GMO products.

• Environmental

• Agriculture: Crops

• Veterinary

• Food Safety and Qulaity