Biotechnology 7.4 HW

What is the purpose of a spectrometer?

• To measure the intensity of light that passes through a sample.

• To combine light into its component wavelengths.

• To emit a unique spectrum of light.

• To measure the amount of light emitted off or by a source.

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NGSS.HS-PS4-1

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Which instrument combines a spectrometer and a photometer?

• Spectrometer

• Photometer

• Spectrophotometer

• Fluorescence Spectrometer

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NGSS.HS-PS4-3

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What does the Nucleic dotMetricTM assay estimate the concentration of?

• 10 µL DNA samples

• 1 µL DNA samples

• 100 µL DNA samples

• 50 µL DNA samples

In the context of spectroscopic characterization, what does a sample emit?

• Its own unique spectrum of light

• Intense UV light

• Random wavelengths of light

• No light at all

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NGSS.HS-PS4-1

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Which of the following is not a typical application of spectrometers?

• Measuring the amount of gold in coins and jewelry

• Looking for chemical pollutants in the environment

• Analyzing food and beverage quality

• Diagnosing skin cancer

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What distinguishes a spectrometer from a spectrophotometer?

• A spectrometer measures specific wavelengths of light.

• A spectrophotometer measures the light emitted by a source.

• A spectrophotometer combines a spectrometer and a photometer.

• A spectrometer measures the intensity of light that passes through a sample.

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NGSS.HS-PS4-3

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What type of spectrometer is used to detect a compound by its molecular weight?

• Infrared Spectrophotometer

• Mass Spectrometer

• Fluorescence Spectrometer

• Nuclear Magnetic Resonance (NMR) Spectroscopy

How does a mass spectrometer work?

• By measuring the intensity of light that passes through a sample.

• By converting molecules in the sample into ions and analyzing their charge-to-mass ratio.

• By emitting its own unique spectrum of light.

• By measuring the fluorescence light emitted from molecules.

What type of spectroscopy uses IR light to study chemical compounds?

• Fluorescence Spectroscopy

• Nuclear Magnetic Resonance (NMR) Spectroscopy

• Fourier-transform Infrared Spectroscopy (FTIR)

• Mass Spectrometry

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In which applications is fluorescence spectroscopy commonly used?

• Analyzing food quality

• Studying protein structural changes

• Measuring the intensity of light that passes through a sample

• Analyzing chemical pollutants in the environment