ABO blood grouping

What is the purpose of blood grouping?

• To identify susceptibility to various diseases

• To determine paternity disputes

• To check routine health

• To avoid reactions of mismatched transfusion

Which blood group is considered the universal donor?

• Group AB

• Group B

• Group A

• Group O

What is the advantage of the slide grouping technique?

• It is highly sensitive

• It can be used during camps

• It is a routine test

• It allows longer incubation of antigen and antibody mixture

What is the recommended method for ABO grouping?

• Tube technique

• Slide test

• Microplate

• Test Tube Method

What is the procedure for cross-matching in blood transfusion?

• Add two drops of anti A, anti B, anti D in three different tubes

• Prepare 2-5% suspension of test sample in normal saline

• Add one drop of cell suspension (A cell to A tube, B cell to B tube and one drop of O cell to O tube

• Place 2 drops of recipient's serum and add washed donor red cell suspension

What is the interpretation if there is no agglutination and hemolysis in cross-matching?

• Mismatched

• Matched

• Negative result

• Positive result

What is the purpose of the tube method in ABO typing?

• To avoid reactions of mismatched transfusion

• To detect weaker antigen/antibody

• To determine the antigen expressed

• To determine the presence of antibody

What is the reciprocal relationship between ABO antigens and antibodies?

• Antigens on RBCs: None, Antibody in plasma/serum: Anti-A, Anti-B

• Antigens on RBCs: AB, Antibody in plasma/serum: None

• Antigens on RBCs: B, Antibody in plasma/serum: Anti-A

• Antigens on RBCs: A, Antibody in plasma/serum: Anti-B

What is the disadvantage of the slide grouping technique?

• It is a routine test

• It is highly sensitive

• Drying of reaction giving false positive results

• It can be used during camps

What is the interpretation if there is either agglutination or hemolysis in cross-matching?

• Positive result

• Mismatched

• Matched

• Negative result