Quality-HPLC (reading)

What is the principle of HPLC?

• Separation of compounds based on their molecular weight

• Separation of compounds based on differential partitioning between a stationary phase and a liquid mobile phase

• Separation of compounds based on their color

• Separation of compounds based on their boiling point

What is the stationary phase in HPLC?

• Solid support with specific properties coated in column format

• Liquid solvent or solvent mixture

• Volatile and thermally unstable compounds

• Gas phase

What is the mobile phase in HPLC?

• Gas phase

• Solid support with specific properties coated in column format

• Volatile and thermally unstable compounds

• Liquid solvent or solvent mixture

What is the separation mechanism in HPLC based on?

• Compounds separate based on their molecular weight

• Compounds separate based on their color

• Compounds partition between stationary phase and liquid mobile phase based on their interactions with both phases

• Compounds separate based on their boiling point

What is the purpose of HPLC?

• Analyzing a wide range of compounds including polar and non-polar substances

• Analyzing only non-polar substances

• Analyzing only polar substances

• Analyzing compounds based on their color

What is the advantage of HPLC?

• Versatile and applicable to various compound types

• Qualitative analysis capabilities

• Applicable to only one type of compound

• Low resolution and separation efficiency

What is the limitation of HPLC?

• Relatively slow compared to gas chromatography

• Does not require specialized equipment and maintenance

• Relatively fast compared to gas chromatography

• Ideal for volatile and thermally unstable compounds

What is the detection method used in HPLC?

• Only refractive index detector

• Only UV-Vis absorbance detector

• Only fluorescence detector

• Various detectors: UV-Vis absorbance, fluorescence, refractive index, mass spectrometry (MS), etc.

What is the parameter used to determine a compound's travel time in HPLC?

• Retention Time: Time taken for a compound to travel from injection to detection

• Boiling Time: Time taken for a compound to boil

• Molecular Weight Time: Time taken for a compound to change its molecular weight

• Color Time: Time taken for a compound to change color

What is the separation time in HPLC?

• Very short, typically a few seconds

• Very fast, typically a few milliseconds

• Very long, typically several days

• Moderate to long, typically from minutes to an hour or more