Immunostaining

What is the difference between direct and indirect immunostaining?

• Direct uses a primary/secondary antibody; indirect uses a conjugated antibody

• Direct uses a conjugated antibody; indirect uses a primary/secondary

• Direct uses fluorescent antibodies, indirect uses HRP-based detection

• Direct uses HRP-based detection; indirect uses fluorescent antibodies

Which of the following methods uses an HRP-based detection?

• Immunofluorescence

• Immunostaining

• Immunohistochemistry

• Immunocytochemistry

How do you validate your antibody? Select all that apply.

• Negative control

• Check the literature

• Check manufacturer's website

• Positive control

Which of the following is NOT a method for analyzing protein?

• Mass spectrometry

• Flow cytometry

• Southern blotting

• Western blotting

How is immunofluorescence used in medicine?

• To test for rabies

• To detect different immune cells

• Anti-nuclear antibody test

• To look for mutations in a patient's tumor