BIOLOGY

What is an application of CRISPR cas 9 technology?

• to edit a faulty allele of a gene in a person with a severe inherited disease.

• to open a cafe

• to cure an individual from cancer

• to activate or repress a gene

Which of the following correctly describes how CRISPR-Cas 9 changes DNA?

• cutting DNA up to create a new chromosome.

• substituting nucleotides by cutting and ligating the sugar phosphate backbone

• by cutting an entire gene out of a cell and replacing it with an entirely new gene

• making a cut in the DNA and relying on DNA repair machinery to introduce a mutation.

what are the two major features of the CRIPSR complex?

• guide RNA

• CAS 9

• CRISPR cas 9

• Deoxyribonucleic acid

What is the role of the PAM sequence?

Answer explanation

The Protospacer Adjacent Motif ensures the CRISPR-Cas9 does not target the bacteria’s own DNA.​

what does palindromic refer to?

Answer explanation

Palindromic refers to it being the same when read from a 5’ to 3’ or 3’ to 5’.

what are spacers and what do they add to the CRISPR complex?

Answer explanation

short sequences of DNA obtained from invading bacteriophages that are added into the CRISPR sequence

CRISPR is found in prokaryotes

• True

• FALSE

How does CRISPR change DNA?

Answer explanation

making a cut in the DNA and relying on DNA repair machinery to introduce a mutation.

What is attenuation and how does it regulate trp levels?

Answer explanation

Attenuation is a process that uses tRNA levels to control the trp operon,it regulates TRP level at an RNA level

When trp levels are high domains 3 and 4 don't base pair together?

• True

• False