qRT-PCR Quiz

What is the difference between qRT-PCR and PCR?

• qRT-PCR uses cDNA and PCR uses gDNA

• qRT-PCR uses gDNA and PCR uses cDNA

• qRT-PCR uses RNA and PCR uses cDNA

• qRT-PCR uses gDNA and PCR uses RNA

What enzyme is used in a qRT-PCR reaction?

• Ligase

• Reverse transcriptase

• Taq Polymerase

• Dispase

What is the purpose of a noRT control?

• To check for RNA contamination in the reagents

• To check for gDNA contamination in the reagents

• To make sure the cDNA synthesis worked

• To make sure that the qRT-PCR reaction worked

What is the purpose of a no template control?

• To make sure that the cDNA synthesis worked

• To make sure that the qRT-PCR worked

• To make sure that the reagents are not contaminated with anything

• To make sure the reaction worked

What does Ct stand for in qPCR?

• Cycling taq

• Cycle threshold

• Computed threshold

• Computed tomography