An account of microbiology lab and use of microscope

Microscopy is

• To reduce the size of object

• To enlarge the object

Principle of bright field microscopy is

• Visible light passes through the specimens only results in magnification of object

• Visible light passes through the lenses only results in object magnification

• Light passes through the specimen and lenses results in object magnification

In most light microscopes, the objective lens, magnifies objects 100X (times), and the ocular lens, magnifies 10X.

Total magnification of organism is

• 1000

• 10000

• 10

• 100

Ocular lens has magnification of

• 10x

• 100x

• 40x

• 5x

Immersion oil functions

• To achieve the level of resolution desired with 1000X magnification oil

• Used to fill the space between the objective lens and the glass slide onto which the specimen has been affixed

• Oil enhances resolution by preventing light rays from dispersing and changing wavelength after passing through the specimen

• All of above

• None of the above

Dark field microscopy is used to visualise

• E.coli

• Staphylococcus

• Trepenoma

Example of flouroscent staining is

• Auramine rhodamine

• Gram stain

• Zn stain