Transformation - iGEM Thessaloniki

After the heat shock we incubate the cells on ice to recover for:

• 2 minutes

• 30 minutes

• 45 seconds

• 1 hour

We add CaCl2 because Cl increases the permeability of the membrane

• True

• False

The temperature of the water bath for the heat shock has to be:

(E.Coli)

• 30°C

• 50°C

• 42°C

Transformation is applicable to eukaryotic cells

• True

• False

Answer explanation

Transfection is the "tranformtion analogue" of eukaryotic cells

To endure the heat shock treatment the cells used are in:

• The log phase of growth

• The lag phase of growth

• The stationary phase of growth

We use IPTG (isopropyl - β- D-1- thiogalactopyranoside) to :

• makes the membranes permeable

• trigger the transcription of the lac operon

• recover the cells after the heat shock

Answer explanation

After we apply high- voltage electric pulse to the cells we incubate them in ice to recover :

• True

• False

In blue-white screening, the blue colonies are bacterias

• with recombinant plasmids

• with non-recombinant plasmids

• with no plasmids

Which methods can be used to detect recombinant vectors?

• Western blot

• Restriction enzymes + electrophoresis

• Plasmid with lethal gene

• Electroporation

Sanger sequencing is the most accurate method to detect recombinant vectors

• True

• False