Gram Staining Technique Quiz

What is the first step in performing gram staining?

• Transfer a colony of bacteria using a metal loop

• Add a drop of sterile water to the circle

• Draw a circle on the slide

• Label the microscope slide with your initials

What is the purpose of flaming the metal inoculation loop?

• To sterilize it

• To transfer bacteria

• To evaporate the liquid

• To cool it down

What is the purpose of heat fixing the bacteria to the slide?

• To evaporate the liquid

• To cool down the bacteria

• To attach the bacteria to the slide

• To sterilize the slide

What is the purpose of adding Crystal Violet stain to the bacteria?

• To stain the bacteria

• To rinse the bacteria

• To cool down the bacteria

• To sterilize the bacteria

What is the purpose of adding grams iodine to cover the bacteria?

• To cover the bacteria

• To rinse the bacteria

• To cool down the bacteria

• To sterilize the bacteria

What is the purpose of the decolorizer in gram staining?

• To sterilize the bacteria

• To cool down the bacteria

• To add color to the bacteria

• To remove the stain from some bacteria

What is the purpose of adding safranin stain to cover the bacteria?

• To stain the bacteria

• To rinse the bacteria

• To sterilize the bacteria

• To cool down the bacteria

What should be done after the slide is completely dry in gram staining?

• Rinse the slide in decolorizer

• Flame the slide again

• Add more water to the slide

• Observe the sample under a microscope