Chromatography Quiz

What is the purpose of preparative chromatography?

• To analyze the molecular weight of proteins

• To separate the components of a mixture for purification

• To determine the existence of analytes

• To measure smaller amounts of material

What is the stationary phase in chromatography?

• The substance to be separated during chromatography

• The substance fixed in place for the chromatography procedure

• The phase that moves in a definite direction

• The solvent that carries the analyte

Which chromatography technique is used for the separation of amino acids, proteins, and carbohydrates?

• Thin Layer Chromatography (TLC)

• Column Chromatography

• Gel Filtration Chromatography

• Gas Liquid Chromatography

What is the mobile phase in gas chromatography (GC)?

• A solvent on a solid support

• A solvent in a liquid chromatography (LC)

• A gas

• A packing of porous particles

What is the purpose of high-performance liquid chromatography (HPLC)?

• To separate, identify, and quantify each component in a mixture

• To determine the molecular weight of proteins

• To check the purity of organic compounds

• To monitor the extent of a reaction

What is the eluent in chromatography?

• A list of solvents ranked according to their eluting power

• A stationary phase that is immobilized on the support particles

• The solvent that carries the analyte

• The mobile phase leaving the column

What is the purpose of analytical chromatography?

• To separate the components of a mixture for purification

• To analyze the molecular weight of proteins

• To determine the existence and concentration of analytes in a sample

• To measure the retention time of analyte

What is the detector in chromatography used for?

• To separate, identify, and quantify each component in a mixture

• For qualitative and quantitative detection of analytes after separation

• To determine the molecular weight of proteins

• To check the purity of organic compounds

What is the purpose of size-exclusion chromatography (SEC)?

• To separate analytes based on their size

• To check the purity of organic compounds

• To monitor the extent of a reaction

• To separate, identify, and quantify each component in a mixture

What is the purpose of thin-layer chromatography (TLC)?

• To check the purity of organic compounds

• To separate the components of a mixture for purification

• To determine the existence and concentration of analytes in a sample

• To monitor the extent of a reaction