Enzyme Immobilization Quiz

What are the primary advantages of using immobilized enzymes in industrial applications?

• Reduced specificity and increased reaction time

• Increased specificity and the ability to be reused

• Decreased efficiency and throughput

• All of the above

Which factor does NOT lead to enzyme deactivation?

• Optimal temperature

• Fluid forces

• Chemical agents like urea

• Irradiation

What is the main purpose of enzyme stabilization?

• To decrease the efficiency of enzymes

• To maintain enzyme activity over time

• To enhance the reaction conditions

• To reduce the specificity of enzymes

Which method is NOT a chemical method of enzyme immobilization?

• Enzyme cross-linking by multifunctional reagents

• Cross-linked enzyme matrix

• Enzyme entrapment within insoluble gel matrix

• Enzyme attachment to the matrix by covalent bonds

What is the Damköhler number used for in the context of immobilized enzymes?

• To measure the cost of enzyme immobilization

• To assess the efficiency of immobilized enzyme systems

• To determine the toxicity of immobilization agents

• To identify the enzyme's active site

What does microencapsulation in enzyme immobilization involve?

• Formation of covalent bonds

• Cross-linking of enzyme molecules

• Creating microscopic hollow spheres

• All of the above

Which material is NOT typically used for enzyme immobilization?

• Calcium alginate

• Agar

• Polyacrylamide

• Polyethylene

What is a major disadvantage of enzyme entrapment?

• Increased reaction rate

• Enzyme leakage into the solution

• Enhanced control of microenvironmental conditions

• Increased enzyme stability

Which is not a method to overcome the disadvantages of enzyme entrapment?

• Using competitive inhibitors

• Adjusting membrane molecular weight cutoff

• Reducing the particle size of matrices

• Increasing the diffusion limitations

What is the effect of diffusional limitations on immobilized enzymes?

• They increase the rate of reaction

• They have no impact on the enzyme's activity

• They can lead to a decrease in overall activity

• They enhance the binding of substrate to the enzyme