Transformation Quiz (Molecular Genetics)

What shape is a plasmid?

• small and circular

• large and circular

• small and square

• small and triangular

(a)   is the method of shocking a cell with electricity to make the cell membranes more permeable to DNA.

• Electroporation
• Calcium Chloride
• Heat Shock
• Electrical Manipulation

​ (a)   is the method used to cause chemically-competent cells to uptake DNA after heat shock.

• Calcium Chloride/Heat Shock
• Electroporation
• Temperature Manipulation
• Chlorination Manipulation

What is beta-lactamase enzyme (bla) resistant to?

• penicillin

• amoxicillin

• ampicillin

• arabinose

What is the first step of the Transformation Procedure?

• incubate (on ice)

• collect cells

• heat shock

• add nutrient broth

Cells with pGLO will​ (a)  

• grow and glow
• shrink and glow
• grow and be dull
• shrink and be dull

What does Arabinose control?

• Green Fluorescent Protein (GFP)

• Arabinose

• Beta-lactmase enzyme (bla)

• araC

What does ori allow?

• plasmid replication

• uptake of GFP

• resistance of ampicillin

• uptake of calcium

What animal are we transforming genes from?

• Jellyfish

• Chicken

• Cat

• Shark

Does a plasmid replicate autonomously?

• Yes

• No

• Maybe

• Sometimes