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Reverse Grouping

• Agglutinogens

• Antibodies

AB X OO Genotypes has an offspring phenotypes of

• A Offspring Phenotype

• AB Offspring Phenotype

Antigens in ABO Blood Group System:

• Present in Kidneys

• Found in CSF

Formation of ABH Antigen

• Se gene – Chromosome 19

• H gene – Chromosome 19

Acquired B Phenomenon

• Associated with elderly patients

• Associated with strains of E.coli O86 C. tertium, P. vulgaris O19

Blood Group System

• MNS – Chromosome 4

• LUTHERAN – Chromosome 19

Bombay phenotype

• Agglutinates in Anti-H antisera

• No agglutination reaction in Anti-A and Anti-B antisera

Group 4 Discrepancies Resolution

• If for serum = Reagent RBC and serum can be warmed to 37 degrees Celsius

• Not successful forward typing = Treat patient’s RBC with 0.01M dithiothreitol to dispersed agglutination reaction

A2 phenotype

• Anti-A Reagent = (+)

• Anti-A1 Lectin Reagent = (+)

A1 phenotype in relation with the substances present in saliva:

• A Antigen

• H Antigen