Review MPB 4

The goal of transference techniques is...

• to separate RNA molecules

• to separate DNA molecules

• to separate and characterize nucleic acids or proteins

• to characterize proteins

In transference techniques, the bands you are interested in are...

• The bands that appear in the gel

• The bands you can transfer to the membrane

• The bands identified with the probe or antibody

• none of the other answers is correct

In a Northern blot you are analyzing...

• DNA

• RNA

• Proteins

• Antibodies

Genomic libraries constructed by cloning is the only way to sequence eukaryotic genomes

• True

• False

During the construction of a genomic library it does not matter the kind of vector you are using as all types can be used

• True

• False

Reverse transcription is a process that....

• creates dsDNA from cDNA

• creates ssDNA from mRNA

• creates dsDNA from mRNA

• nicks circular dsDNA

Sequence coverage is ...

• The averaged number of bp found in the clones of a genomic library

• The averaged number of inserts generated in a genomic library

• The averaged number of times sequencing topics appear in social media

• The averaged number of times a sequence appears in a genomic library

Mark the options corresponding to library screening strategies:

• DNA hybridization with probes

• Screening with antibodies

• Functional completation

• All are correct answers

The golden stardard of sequencing during more than 30 years has been

• Nanopore sequencing

• Sanger sequencing

• Illumina sequencing

• PacBio sequencing

What is the key principle used in Sanger sequencing?

• The lack of a hydroxyl group in dideoxynucleotides

• The presence of a hydroxyl group in deoxyribonucleotides

• The use of electrophoresis

• The presence of a fluorescent dye linked to the four deoxyribonucleotides