Repaso MPB clase3

Ligases are enzymes that...

• degrade exogenous DNA

• catalyze the hydrogen bond bewteen complementary bases

• cut the dsDNA

• catalyze a phosphodiester bond

One of the key characteristics that vectors must have is the capacity of replication in the host cell

• True

• False

A plasmid is able to replicate only during the division of the cell

• True

• False

• A plasmid does not replicate

Which part of a virus must be removed to use it as a vector during gene therapy?

• Its entire genome

• The genes encoding the infectious and pathogenic capacity

• Its capside

• The genes encoded by its plasmid

The polylinker is the sequence of a vector where

• the marker gene is located

• the origin of replication is located

• the recognition site of restriction enzymes is located

• the ligases link to dsDNA

Electroporation is...

• a physical method to introduce DNA into host cells

• what you will suffer if you fail the final exam

• a method to separate DNA molecules

• a chemical method to introduce DNA into host cells

Agrobacterium-mediated gene transfer, that uses a modified Ti plasmid as vector is used to modify

• all kinds of eukaryotic cells

• bacteria and yeasts

• plants and fungi

• bacteria and plants

There are two ways to ensure the stability of recombinant DNA in host cells. One is the autoreplicative capacity of vectors. The other one is...

• the capacity of duplication only during cell division

• repeating the cloning sequence

• the capacity to recombine with the host cell genome

Which characteristic of a vector allows the differentation between cells that have adquired the recombinant DNA from those that have not?

• the marker

• the origin of replication

• the lenght of the insert

• the polylinker

What are the sticky ends?

• your fingers after eating nachos

• single-stranded complementary ends on double-stranded DNA

• double-stranded ends on double-stranded DNA

• strand ends on RNA