BIOCHEMICAL TESTING (OTHERS)

Differentiate microorganisms based on the ability to use acetamide as the sole source of carbon

• Acetamide Utilization
• Acetate Utilization
• l-Alanine-7-amido-4-methylcourmarin (Gram-Sure)
• Bacitracin Susceptibility

Differentiate organisms based on ability to use acetate as the sole source of carbon. Generally used to differentiate Shigella spp. from Escherichia coli.

• Acetamide Utilization
• Acetate Utilization
• l-Alanine-7-amido-4-methylcourmarin (Gram-Sure)
• Bacitracin Susceptibility

This test is used in conjunction with the Gram stain to distinguish aerobic grampositive rods or coccobacilli that may appear gramnegative or gramvariable

• Acetamide Utilization
• Acetate Utilization
• l-Alanine-7-amido-4-methylcourmarin (Gram-Sure)
• Bacitracin Susceptibility

This test is used for presumptive identification and differentiation of beta-hemolytic group A streptococci (Streptococcus pyogenes– susceptible) from other betahemolytic streptococci. It is also used to distinguish staphylococci species (resistant) from micrococci (susceptible)

• Acetamide Utilization
• Acetate Utilization
• l-Alanine-7-amido-4-methylcourmarin (Gram-Sure)
• Bacitracin Susceptibility

Acetamide Utilization: The production of ammonia results in an _____ pH, causing the medium to change color from green to royal blue.

• alkaline
• acidic

Acetamide Utilization: Positive Quality Control — growth; blue color

• Pseudomonas aeruginosa (ATCC 27853)
• Escherichia coli (ATCC 25922)

Acetamide Utilization: Negative Quality Control — no growth; green color

• Pseudomonas aeruginosa (ATCC 27853)
• Escherichia coli (ATCC 25922)

Acetate Utilization: Positive Quality Control — growth; blue color

• Escherichia coli
• Shigella sonnei

Acetate Utilization: Negative Quality Control — small amount of growth; green

• Escherichia coli
• Shigella sonnei

Acetate Utilization: Some strains of E. coli may use acetate at a very slow rate or not at all, resulting in a false negative reaction in the identification process.

• TRUE
• FALSE