16Mar.Genomics

Hows the course been to you so far?

• pretty bad

• im struggling to keep up

• im fine; doing well

• enjoying it

What biological process allows the diversity of cell types and life forms?

• DNA replication

• Cellular respiration

• Gene regulation

• Protein synthesis

Answer explanation

While DNA replication, cellular respiration, and protein synthesis are essential processes for cellular function, it is gene regulation that specifically enables the diversity of cell types and life forms. By selectively turning genes on and off, different cell types can develop, even though they share the same genome.

What does the term "multipotency potential" refer to in the context of cells?

• The ability of a cell to produce energy

• The capacity of a cell to differentiate into multiple cell types

• The rate at which a cell divides and reproduces

• The capacity of a cell to communicate with other cells

Answer explanation

Multipotency potential refers to the capacity of certain cells, such as stem cells, to differentiate into multiple cell types, giving rise to various specialized cells in the body.

What are the key regulators responsible for determining which genes are turned on and off within a cell? (a)  

• Transcription factors
• Mitochondria
• Ribosomes
• Cell membranes

Answer explanation

Transcription factors are proteins that play a crucial role in gene regulation. They control the process of turning genes on and off by binding to specific DNA sequences, thereby influencing the expression of various genes within a cell.

Which of the following genomic techniques has provided insights into the heterogeneity within hematopoietic stem cell (HSC) populations?

(a)  

• Single-cell RNA sequencing (scRNA-seq)
• Chromatin immunoprecipitation sequencing (ChIP-seq)
• Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq)
• Whole-genome sequencing (WGS)

Which technique is used to study chromatin accessibility and remodeling during hematopoietic stem cell (HSC) differentiation?

• Chromatin immunoprecipitation sequencing (ChIP-seq)

• Single-cell RNA sequencing (scRNA-seq)

• Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq)

• Whole-genome sequencing (WGS)

What is the primary purpose of HSC transplantation in treating various blood disorders?

• To improve donor-recipient matching

• To provide a curative therapy

• To study the role of HSCs in disease

• To understand HSC self-renewal and differentiation

Which gene editing technology has enabled targeted correction of disease-causing mutations in patient-derived hematopoietic stem cells (HSCs)?

• TALENs

• Zinc-finger nucleases

• CRISPR/Cas9

• Homing endonucleases