Mol Bio Quiz6

Match the following enzymes with their function(s)

• Degrade protein

• Proteinase

• Synthesize cDNA from RNA template

• DNA polymerase

• Degrade RNA

• Rnase

• Synthesize new strands of RNA

• RNA polymerase

• Synthesize new strands of DNA

• Reverse transcriptase

The DNA can be denatured by

• Chemical Treatment

• Heat Treatment

• Both A and B

• Neither A nor B

Which of the following is NOT a benefit of using model organisms in scientific research

• They are easily genetically manipulated

• They have well-characterized developmental pathways

• They are inexpensive to maintain and reproduce

• They provide a perfect representation of human biology

Which of the following is not a post-transcriptional modification process

• Deamination

• Capping

• Polyadenylation

• Splicing

Which type of point mutation would result in the substitution of an amino acid by a stop codon

• Frameshift

• Nonsense

• Missense

• Silent

You amplified the gene coding for a digestive protein using PCR, cloned it into a plasmid and expressed it in bacterial cell to produce the protein of interest. Your protein expression experiments kept failing and you decided to sequence the PCR product to check if you had amplified the region of interest. Turns out there is a non-sense mutation that occured in the gene you amplified. How does this explain your protein expression results?

• The nonsense mutation impacted the host bacteria and stopped protein production

• The nonsense mutation stopped the PCR reaction and hence the gene was not amplified

• The nonsense mutation created nonsense and doesn't make sense

• The nonsense mutation led to a stop codon in the gene sequence you cloned and hence the protein cannot be produced