MCBI 603

Which of the following is the basic requirement of PCR reaction

• Two oligonucleotide primers

• DNA fragment to be amplified

• A heat-stable DNA polymerase

• All of the above

What is the process of binding of primer to the denatured strand called?

• Annealing

• Renaturation

• Denaturation

• None of the above

At what temperature does denaturation of DNA double helix takes place?

• 54°C

• 74°C

• 94°C

• 60°C

How many DNA duplexes are obtained from one DNA duplex after 4 cycles of PCR?

• 8

• 4

• 32

• 16

What are the base-pairing rules in DNA?

• A-C, G-T

• A-U, G-C

• A-T, G-C

• A-C, G-U

You decide to use PCR to determine if you have Cystic Fibrosis, a genetic disorder. You then decide to also check if you are at risk for developing Alzheimers, another genetic disorder. What PCR "ingredient" would be different in these two tests?

• Template DNA

• Nucleotides

• Taq DNA polymerase

• Primers

What is the purpose of gel eectrophoresis

• helps cut DNA

• count the genes in DNA

• separates DNA based on size

• allows for an exact replicated organism

Buffers used in electrophoresis....

• conducts electricity

• removes salts

• add extension energy

• dissolve excess DNA

Which of the following factors will not affect the rate of migration of DNA in agarose gels?

• DNA concentration

• Agarose concentration

• Voltage

• DNA conformation

Ethidium bromide is a stain that specifically binds to DNA or RNA and appears ______ if viewed under ultraviolet light

• red

• yellow

• orange

• blue