Lab 3 quiz

In assessment of RNA purity, what is the desirable ratio of absorbance at 260 nm and 280 nm for pure sample of RNA?

• 0.9 - 1.1

• 1.2 - 1.4

• 1.5 - 1.7

• 2.0-2.2

Which of the following factor does not influence electrophoretic mobility

• Molecular weight

• Shape of the molecule

• Charge of the molecule

• Stereochemistry of the molecule

Which of the following factors can affect the accuracy of DNA quantification?

• The age of the DNA sample

• The presence of contaminants in the sample

• The instrument used for quantification

• All of these

Which is the most likely outcome if a student adds their DNA sample to the first well containing the DNA ladder in agarose gel electrophoresis?

• Slower DNA ladder migration due to interference from the student's DNA

• Faster student DNA migration due to the presence of the ladder

• No separation, ladder and student's DNA migrate as a single band

• DNA ladder is destroyed due to the student's DNA presence

Which of the following cannot be used as blank for DNA quantification

• Bromophenol blue

• Elution buffer

• TE Buffer

• Distilled water

Which factor determines the resultant 260/280 ratio of nucleic acid

• Weighed average of 4 nucleotides

• Weighed average of Guanine and cytosine

• Average of phosphates and sugars

• None of these

Which of the following is used as tracking dye in agarose electrophoresis

• Ethidium bromide

• Bromophenol blue

• Glycerol

• Both a and b

You are preparing an agarose gel to run your PCR products on. Your products are 800bp and 1.2kb. From the list below, what mass of agarose would be appropriate if you plan to make up 50ml of gel?

• 1-1.2 gm

• 1.5-2.0 gm

• 0.35-0.40

• 0.5 gm

Following is the paternity test report of the child. Identify the correct option

• Dad 1

• Dad 2

• Dad 3

• None of the above

The attachment between the base and sugar in a nucleotide is through ______ bond.

• hydrogen

• peptide

• phosphodiester

• glycosidic