Quiz 7 (Lec. 12 & 13)

What force(s) do settling chambers use to separate solid and liquid material?

• Flow

• Applied force

• Gravity

• Flow & Gravity

Which of the following techniques is involved in clarification and isolation?

• Chromatography

• Precipitation

• Filtration

• All of the above

The most common filtration method is to separate based on _____.

• charge

• size

• mass

• viscosity

________ filtration collects large build-up on the membrane surface and within the filter matrix.

• Tangential

• Depth

• Normal flow

• Centrifugation

Which of the following is an example of a centrifuge filtration method used in the laboratory?

• Sample concentration

• Purification

• Size selection

• All of the above

Which of the following is not a disadvantage associated with cell disruption?

• Significantly reduced bioproduct recovery/yield

• Natural incremental loss of the bioproduct

• Unwanted cellular material is also released into the media

• Increases time & materials required

What cell disruption method is best suited for a startup concerned with cost of supplies and operational expenses?

• Chemical

• Non-chemical/mechanical

• Enzymatic digestion

• Freeze/thaw cycles

Tags

NGSS.HS-ETS1-3

What variable(s) is measured from the filtrate that exit chromatography columns?

• Ion concentration/Conductivity

• Protein concentration/Absorbance (A280)

• Elution time

• Ion & protein concentration

What are the steps involved in affinity chromatography? (order matters)

• Sample application, elution, equilibration

• Equilibration, sample application, elution

• Equilibration, sample application, elution, re-equilibration

• Sample application, elution, equilibration, re-equilibration

Which of the following is an advantage associated with affinity chromatography?

• Cheaper materials

• Sensitivity; high bioproduct yield

• Specificity; up to 1000 fold purification

• Reproducible; ease of use