Gel Electrophoresis

Electrophoresis is a s_________ technique

• substitution

• separation

• symbiotic

• segregation

Electrophoresis can be used to separate mixtures of...?

• DNA

• proteins

• amino acids

• lipids

Like chromatography, gel electrophoresis has a mobile phase and a stationary phase. Which of the following is the stationary phase?

• The electrophoresis tank

• The gel

• The DNA fragments

• The power supply

What 'a' is the electrophoresis gel made of?

• arginine

• aspartame

• agarose

• aspergillus

What type of organism is the agarose extracted from?

• jellyfish

• sea urchins

• starfish

• seaweed

Which of the following is the mobile phase in gel electrophoresis?

• The mixture of DNA/proteins/amino acids

• The current provided by the power supply

• DNA probes

• Restriction enzymes

How do we describe the charge on a DNA molecule?

• negative

• positive

• polar

• non-polar

Which part of the DNA molecule makes it negatively charged?

• The nitrogenous bases

• The ribose sugars

• The phosphate groups

• The deoxyribose sugars

A loading buffer is added to the DNA mixture(s) before it is pipetted into the well(s). What is/are the functions of the loading buffer?

• to increase the density of the sample

• to decrease the density of the sample

• to be able to see if the electrophoresis has worked

• to identify the bands of DNA

The mixture(s) of DNA fragments is/are pipetted into (a) wells in the electrophoresis slab. What is pipetted into the final well?

• DNA size standards

• RNA probes

• DNA probes

• restriction enzymes