serological test

what is serology?

• science that attempts to detect signs of infection in a patient's serum such as Ab for a specific microbe.

• When a soluble antigen reacts with its specific antibody at an optimum temperature and PH in the presence of electrolyte.

• process of a combination of immuno-diffusion and electrophoresis.

There are 2 types of Immunofluorescence are direct and Passive

• Ture

• False

Component of Ring precipitation test are:

(more than one choice is correct)

• Material from patient ( unknown Ag )

• Erythrocytes from sheep.

• Specific precipitation serum ( known Ab )

• Hemolytic serum ( present Ab against erythrocytes from sheep )

Steps of Western blot test

(more than one choice is correct)

• mixing test Ag and Ab with complement

• Proteins detection

• Proteins transfer

• Sample preparation

Material required in Immunoelectrophoresis is

• Buffer

• Coombs test

• Fluorescent microscope

• Haemolytic serum

Agglutination tests is used in

• Detection of Streptococcus pyogenes

• Bacteriological method for identification of pure culture

• Express diagnosis of infection disease by detection of immunoglobulin levels in the serum of the patient.