CRISPR Lab quiz

The enzyme portion of this complex is called ________.

• CRISPR

• Replicase

• Lysozyme

• Cas9

What type of RNA is used as a GPS of sorts, to find or locate specific genes?

• mRNA

• gRNA

• tRNA

• rRNA

After Cas9 cuts the target DNA, cellular enzymes attempt to repair the break. Which of the following repair mechanisms involves "editing" of the gene with insertion of a desired sequence?

• HDR

• NHEJ

What is the function of sgRNA?

• It is inserted to change the code of lacZ gene

• It cuts DNA

• It tells cas9 where to cut

• It creates a blue pigment

Why is HDR a better repair system than NHEJ? (2 correct answers)

• NHEJ can cause mutations

• HDR can add desired new DNA

• HDR is NOT better than NHEJ

• HDR causes random deletions

What is the difference between the pLZDonor and pLZDonorGuide plasmids?

• Only pLZDonor has a Donor DNA Template

• Only pLZDonorGuide has a Donor DNA Template

• Only pLZDonor has sgRNA

• Only pLZDonorGuide has sgRNA

When the lacZ gene is working NORMALLY, what happens?

• B-gal breaks down X-gal

• X-gal breaks down B-gal

• Cas9 cuts the DNA

• A Donor Template DNA is inserted

When the lacZ gene is working NORMALLY, what happens?

• The bacteria appears white

• The bacteria appears green

• The bacteria dies

• The bacteria appears blue

If a bacteria has arabinose, Cas9, and pLZDonorGuide, which color would it appear?

• Blue

• White

• Green

• The bacteria dies

If bacteria has NO arabinose, Cas9, and pLZDonorGuide, which color would appear?

• Blue

• White

• Green

• The bacteria would die