MIC220_Modern techniques for taxonomy

Which of the following is a correct step for hybridisation?

1- add DNA probe that labelled with detector

2 - bind DNA to a membrane

3 - probes hybridise to the target DNA region

4- wash off the excess probes

5 - develop film

• 1, 2, 3, 4, 5

• 2, 1, 3, 4, 5

• 2, 1, 3, 5, 4

• 1, 2, 4, 3, 5

Which of the following statement is NOT true about radioactive probe?

• the nucleic acid is labelled with isotope

• the most common is biotin-streptavidin complex

• the probes can be observed by autoradiography

• the probes can be observed by scintillation

Which blotting technique is to detect RNA?

• Southern blot

• Northern blot

• Western blot

• Eastern blot

Which blotting technique is to detect protein?

• Southern blot

• Northern Blot

• Western Blot

• eastern blot

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing

• It makes the testing simpler by moving the strands into the gel faster

• It restricts the number of base pairs that can be tested in a sample

• It charges the DNA strands

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells

• three quarters away from the starting wells

• farthest from the starting wells

• it depends on how many fragments there are

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit

• the agarose gel in negatively charged

• DNA is positively charged to attracted to the negative end of the unit

• the agarose gel is positively charged

What is the function of the primer?

• To identify the particular region of DNA to be copied by PCR.

• To create DNA nucleotides

• To copy DNA

• To maintain the temperature of the PCR reaction

What is the purpose of the Extension step of PCR?

• To create the primers

• To allow polymerase to create the complementary strands of DNA

• To extend the time it takes to produce DNA

• To allow substrates to copy DNA sequences

Why does PCR require a specific type of polymerase?

• Taq polymerase can withstand the range of temperatures in PCR without denaturing

• Taq polymerase is the most inexpensive type of polymerase

• A specific type of polymerase is unnecessary

• Taq polymerase is the only polymerase used in PCR