BIOTECH- Protein structure & engg.

Active form of chymotrypsinogen contains___________ number of amino acids.

• 244 amino acids interrupted into three peptides.

• 244 amino acids interrupted into two peptides.

• 245 amino acids interrupted into three peptides.

• 246 amino acids interrupted into three peptides.

The main substrate of chymotrypsin includes

• F,W,Y

• F,W,L

• F,W,M

• F,R,Y

Few examples of organophosphate derivates are

• DTT and parathion

• Diazinon and phorate

• marathon and brufen

• Anacin and diazinon

How many fragments would result if the following peptide is digested with enzyme trypsin.

LLYRTWYQBSDKLY

The solvent system used in electrophoretic technique in peptide mapping is

• Ethanol, water, acetic acid

• Butanol, water, ascorbic acid

• Butanol, water, acetic acid

• Butanol, ethanol, acetic acid,

2D gel electrophoresis techniques was proposed by

• Linus pauling

• Max perutz

• V.M.Ingram

• O'Farrel

A single substitution of a nucleotide (SNP) on coding strand of beta globin gene of hemoglobin results in sickle cell anemia. which of the following is TRUE with regard to the above .

 

• Codon GTG  transcribed to  GAG  translated to  GUG

• Codon GAG  transcribed to  GTG  translated to  GAG

• Codon GAG  transcribed to  GTG  translated to  GUG

• Codon GAG  transcribed to  GTG  translated to  GTG

A protein with molecular weight 12,000 Dalton contains 4 charge. The MS detects the protein ions at

• 1204

• 3001

• 301

• 12004

A proteolytic enzyme that digest blocks in arteries following myocardial infarction is

• CD3

• OK-T3

• tPA

• IL2

Which one of the following has been approved for diabetics who develop skin ulcers.

• human growth factor

• humulin

• platelet derived growth factor

• EPO