RECOMBINANT DNA TECHNOLOGY 2.0

1. What is the function of the restriction enzyme for the bacteria themselves?

• to cut their own DNA

• to cut the invaded DNA

• to cut open the plasmid

Choose the restriction enzyme that can do this

• SmaI

• PovII

• BamHI

• HaeIII

The function of restriction enzyme is to recognize and cut the specific sequence called ______________

the same restriction enzyme must be used to produce _________ ends.

the capacity of the DNA fragment is 10 kb. Choose TWO vectors that can be used

• sCOS-1

• pUC18

• λ2001

• pYAC

a DNA from hummingbird cell is cut at 10 recognition sites. How many fragments produced?

• 10

• 20

• 11

• 21

a DNA from humming bird is cut at 10 recognition site. How many fragment contains gene of interest?

• 10

• 11

• 1

• 0

Which of the following gene in the following plasmid having restriction site within to cut open the plasmid?

• Amp^R

• ori

• lacZ

choose the he sequence of sticky/ staggered end based on the diagram.

• GCTTAA

• CTTAA

• TTAA

Choose the enzyme that can be used to seal the seal between plasmid and the inserted DNA fragment is

• Taq polymerase

• DNA ligase

• EcoRI

• SmaI