Translational regulation & protein folding

Which of these are global mechanisms to regulate translation?

• Non-sense Mediated Decay

• Codon usage

• phosphorylation of eIF2 & eIF2B

• mRNA stability

• phosphorylation of 4E-BPs

Secondary structures located at the 5' UTR

• enhance translation

• slow down translation

• have no effect on translation

IRP is an example of a

• Carrier protein

• Repressor protein

• Enhancer protein

miRNA and the RISC regulate translation at what stage

• elongation stage

• initiation stage

• termination stage

Which triggers faster degradation?

• siRNA

• miRNA

Phosphorylation of eIF2 & eIF2B result in

• negative regulation

• positive regulation

Which kinase phosphorylates 4E-BPs?

• PKR (protein kinase R)

• mTOR

• eIF4E

Protein disulfide isomerase is found in the

• nucleus

• ER lumen

• mitochondria

• lysosomes

• cytoplasm

------ have complete complementarity while --------- have mismatches.

• miRNA, siRNA

• siRNA, miRNA

• RISC, RITS

• RITS, RISC

--------- sequences, allow viruses to bypass the cap-dependent mechanism of translation

• IRP

• IRE

• IRES

• ORF